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Accuris 1 Hour Mammalian Genotyping Kit 80 reactions
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- DNA extraction and amplification in 1 hour
- Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
- Single tube - no organic solvents or clean up procedures
- Enhanced Accuris Hot Start Polymerase included
Traditionally, mammalian genotyping protocols have involved Proteinase K
digestion, neutralization, organic extraction and lots of hands on time
to get PCR-ready DNA. The Accuris Mammalian Genotyping Kit is quick and
easy to use - add sample to the proprietary lysis buffer and incubate
for 5-10 minutes, then add the deactivation buffer and incubate for 10
minutes. The crude lysate can them be amplified using fast PCR Accuris
Hot Start Taq Master Mix with red loading dye (included). The kit
contains everything needed - just add sample and primers. Enough for 80 reactions. Sample reactions available on request (shipping cost may apply). This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris High Fidelity DNA Polymerase 200 units
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- 50X higher fidelity than Taq DNA polymerase
- Works with crude DNA sample
- Ideal for cloning, mutagenesis and microarrays
- Optimized buffer system with unique PCR enhancers
For applications requiring highly accurate amplification, choose Accuris
High Fidelity DNA polymerase. Modified for better solubility and higher
activity across a broad range of ionic conditions, this polymerase will
amplify a wide range of targets, including those that are GC or AT rich
as well as crude samples. A 3'-5' proofreading exonuclease activity
and an error rate of 4.55x10-7 makes Accuris High Fidelity DNA
Polymerase the perfect partner for cloning applications. The supplied
5X buffer with dNTPs is optimized for compatibility with a variety of
targets.
Sample reactions available on request (shipping cost may apply). This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris Hot Start Taq Master Mix 200 reactions
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- Exceptional sensitivity for low copy PCR
- Ideal for multiplex PCR and amplification of GC-rich DNA
- Same enhanced features as Accuris Taq Polymerase
- Buffer optimized for fast cycling and reproducibility
Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification. When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Hot Start Taq is provided in a ready to use 2X Master Mix. The Master Mix can be ordered with or without a red gel loading dye (additional costs may apply). Sufficient material for1000 reactions are included in this package. Sample reactions available on request (shipping cost may apply). Red Dye Version available on request (additional costs may apply) This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris Taq Plus Master Mix 200 reactions
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- Higher fidelity for long PCR amplicons, up to 35kb
- Ideal for problematic templates
- Better sensitivity and higher activity for low copy and long PCR
- Enzyme of choice for TA cloning
Accuris Taq Plus, an optimized blend of our Hot Start Taq and a
proofreading polymerase, provides 4x better fidelity than wild-type Taq
and increased enzyme activity. It is the perfect choice for GC and AT
rich templates, low copy number and long PCR. The 3'-5' exonuclease
activity of the proofreading enzyme produces fragments suitable for TA
cloning. Accuris Taq Plus is provided
in a convenient one tube 2X Master Mix.
Set includes Taq Plus for 200 reactions. Sample reactions available on request (shipping cost may apply). This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris 1 Hour Mammalian Genotyping Kit 400 reactions
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- DNA extraction and amplification in 1 hour
- Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
- Single tube - no organic solvents or clean up procedures
- Enhanced Accuris Hot Start Polymerase included
Traditionally, mammalian genotyping protocols have involved Proteinase K
digestion, neutralization, organic extraction and lots of hands on time
to get PCR-ready DNA. The Accuris Mammalian Genotyping Kit is quick and
easy to use - add sample to the proprietary lysis buffer and incubate
for 5-10 minutes, then add the deactivation buffer and incubate for 10
minutes. The crude lysate can them be amplified using fast PCR Accuris
Hot Start Taq Master Mix with red loading dye (included). The kit
contains everything needed - just add sample and primers. Enough for400 reactions. Sample reactions available on request (shipping cost may apply). This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris High Fidelity DNA Polymerase 1000 units
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- 50X higher fidelity than Taq DNA polymerase
- Works with crude DNA sample
- Ideal for cloning, mutagenesis and microarrays
- Optimized buffer system with unique PCR enhancers
For applications requiring highly accurate amplification, choose Accuris
High Fidelity DNA polymerase. Modified for better solubility and higher
activity across a broad range of ionic conditions, this polymerase will
amplify a wide range of targets, including those that are GC or AT rich
as well as crude samples. A 3'-5' proofreading exonuclease activity
and an error rate of 4.55x10-7 makes Accuris High Fidelity DNA
Polymerase the perfect partner for cloning applications. The supplied
5X buffer with dNTPs is optimized for compatibility with a variety of
targets. 1000 units supplied. Sample reactions available on request (shipping cost may apply). This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris Hot Start Taq Master Mix 1000 reactions
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- Exceptional sensitivity for low copy PCR
- Ideal for multiplex PCR and amplification of GC-rich DNA
- Same enhanced features as Accuris Taq Polymerase
- Buffer optimized for fast cycling and reproducibility
Engineered for controlled polymerase activity, Accuris Hot Start Taq is
bound with a monoclonal antibody that blocks enzyme activity. This
allows reactions to be set up at room temperature without the risk of
non-specific amplification. When samples are ready, the reaction mixture
is heated to 95°C to denature the antibody and initiate the reaction. Hot Start Taq is provided in a ready to use 2X Master Mix. The Master Mix can be
ordered with or without a red gel loading dye (additional costs may apply). Sufficient material for1000 reactions are included in this package. Sample reactions available on request (shipping cost may apply). Red Dye Version available on request (additional costs may apply) This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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Accuris Taq Plus Master Mix 1000 reactions
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- Higher fidelity for long PCR amplicons, up to 35kb
- Ideal for problematic templates
- Better sensitivity and higher activity for low copy and long PCR
- Enzyme of choice for TA cloning
Accuris Taq Plus, an optimized blend of our Hot Start Taq and a
proofreading polymerase, provides 4x better fidelity than wild-type Taq
and increased enzyme activity. It is the perfect choice for GC and AT
rich templates, low copy number and long PCR. The 3'-5' exonuclease
activity of the proofreading enzyme produces fragments suitable for TA
cloning. Accuris Taq Plus is provided
in a convenient one tube 2X Master Mix.
Set includes Taq Plus for 1000 reactions. Sample reactions available on request (shipping cost may apply). This product has a flat rate shipping of $33 within the continental US. Ships on ice. Email a question about this item
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