Accuris SmartGlow Pre Stain 1ml 20000x concentration

Product Code: 6235

Our Price: $124.95

Description

Replaces hazardous Ethedium Bromide (EtBr)
Better sensitivity that etBr - Detect as little as 0.1ng of DNA
Two types available - PS Prestain or LD Loading Dye
Excitation by UV Light or blue light
Compatible with Accuris SmartBlue Transilluminator
Ships at ambient temperature (stored at 4C)

SmartGlow PS (Pre Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound the RNA. SmartGlow PS exhibits excitation peaks at 290nm and 490nm, allowing it to be used with UV and blue light.

Protocol:

Prepare 100ml of agarose or polyacrylamide solution
Add 5ul of Smartglow (mechanical pipette strongly suggested) PS stain to the gel solution before pouring gels.
For enhanced results, add the SmartGlow to the running buffer at a ration of 5ul per 100ml. Adding SmartGlow PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid
After electrophoresis is complete, view the gel using a UV or blue light illuminator

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